Web1. Weigh 0.238 g of IPTG (IPTG, GoldBio Catalog # I2481C, [CAS 367-93-1, mw. = 238.3 g/mol]). 2. Add 10 ml sterile H 2 O. Dissolve completely. 3. Prewet a 0.22 µm syringe filter … Webthiogalactopyranoside (IPTG), Sigma Catalog # I5502. 5.1.2.5.1. Kanamycin: Prepare stock solution, filter sterilize using a 0.2 µm syringe filter (B-D # 309602 - Fisher Catalog # 09-719C or equivalent), and store at 2-8°C (for 3 weeks maximum) or-20°C or lower for long-term storage. Add appropriate
IPTG Solution Preparation and Recipe AAT Bioquest
WebIPTG (also known as Isopropyl-β-D-Thiogalactopyranoside) is a molecular biology reagent that functions as an inducer of galactosidase activity by binding to and inhibiting the … WebPrepare 800 mL of distilled water in a suitable container. Add 200 g of IPTG to the solution. Add distilled water until the volume is 1 L. Sterilize by passing it through a 0.22-µm … hattiesburg clinic eye center
Can I prepare the stock solution of reduced glutathione previously …
Web- Greater than 99.8 % purity confirmed by HPLC - Functionally tested in blue/white colony screening Usage: Preparation of stock solution: Dissolve IPTG in water to a final concentration of 200 mg/ml. Sterilize solution using a 0.2 μm filter, dispense in aliquots, and store at –20°C. The sterilized solution is usually stable for several ... WebPrepare a 20 mg/mL stock solution of X-Gal in N,N-dimethylformamide (DMF) or dimethylsulfoxide (DMSO). Sterilization is not required. Store stock solution in glass container protected from light at -20 °C. Solutions may be stored at -20 °C for 6-12 months. If a solution turns pink, it should be discarded. DMF dissolves some plastic materials. WebSpread 40 µL or appropriate amount of stock solution of chromogenic substrate and 10 µL of IPTG solution on LB agar plates using a sterile spreader (addition of IPTG when using product number B3928 is not required as it already contains IPTG). ... Prepare LB agar by weighing appropriate powder medium, agar and water in a sterile flask. ... boots travel vaccinations contact