Unwinding reactions page
WebJun 8, 2024 · The unwinding reactions were proceeded with 2 × 10 −3 m ATP and 2 × 10 −3 m MgCl 2 by using 2 × 10 −6 m DDX21 full‐length or truncations, 0.2 × 10 −6 m 5′‐tailed RNA duplex, and 0.8 × 10 −6 m unlabeled trap RNA. F) RNA unwinding with the full‐length WT and mutant DDX21, ... WebThe unwinding activity of Mtr4p is significantly stimulated by Trf4p/Air2p, but the stimulation of Mtr4p does not depend on ongoing polyadenylation. Nonetheless, polyadenylation …
Unwinding reactions page
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WebDownload. RNA unwinding analyzed by SDS-PAGE. The RNA duplex is annealed as described under " Materials and Methods. " The unwinding reactions are carried out with … WebJan 1, 1999 · reaction parameters on binding and unwinding of the pseudo-or igin *T o whom correspondence should be addressed. T el: +1 212 263 8453; Fax: +1 212 263 8166; Email: borowj01@mcrcr .med.nyu.edu
WebDec 21, 2008 · Trace amounts of HEPES, NP-40 and EDTA are present in the reactions. U4/U6 snRNAs were incubated in the reaction mixture for 5 min at 30°C before initiating unwinding reactions with 2mM ATP. Reactions were performed at 30°C. Aliquots (1 uL) were withdrawn at time points and terminated with 19uL of stop solution (20mM EDTA, … WebApr 1, 1996 · Rate of DNA unwinding as a function of dye concentration. The reactions were performed at 20 °C in standard buffer with 10 µM nucleotide (2.1 nM ends) linear pBR322 DNA, 2 µM SSB protein, 0.5 nM total (0.17 nM functional) RecBCD enzyme, and the indicated concentration of dye. Unwinding was initiated by adding ATP to a final concentration of ...
WebAnalysis of the unwinding reaction was done by SDS–PAGE and autoradiography. We used an intermolecular hybrid with a 17 bp double-stranded region as substrate RNA. Lane 1, ... WebUnwind Reaction Paper. 802 Words4 Pages. Unwind Reaction Paper Imagine a world where a mass amount of children were being aborted not while in the womb, but as teenagers. …
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